Measurement results																																				
26_05_2020_platefrom_20_05_2020																																				
2020-05-26 09:40:10																																				
																																				
Fluorescence 1																																				
Ex 530 nm, Em 590 nm																																				
 																																				
Plate 1																																				
																																				
Reading	avg. time [s]	Blank1 (A01)	Blank1 (A02)	Blank1 (A03)	Blank1 (A04)	Blank1 (A05)	Blank1 (A06)	Blank1 (A07)	Blank1 (A08)	Blank1 (A09)	Blank1 (A10)	Blank1 (B01)	Blank1 (B02)	Blank1 (B03)	Blank1 (B04)	Blank1 (B05)	Blank1 (B06)	Blank1 (B07)	Blank1 (B08)	Blank1 (B09)	Blank1 (B10)	Blank1 (C01)	Blank1 (C02)	Blank1 (C03)	Blank1 (C04)	Blank1 (C05)	Blank1 (C06)	Blank1 (C07)	Blank1 (C08)	Blank1 (C09)	Blank1 (C10)	Blank1 (D01)	Blank1 (D02)	Blank1 (D03)	Blank1 (D04)	Blank1 (D05)
1	0,00	137,9	232,2	70,56	374,0	288,1	447,7	97,10	442,5	12,69	118,6	799,5	2413	2445	2477	2422	2421	1731	2737	1611	2421	1526	1800	2874	2940	2747	2599	1839	1793	1664	772,6	1443	1682	1716	3169	2828
2	60,02	138,3	235,0	73,11	373,2	296,6	460,8	95,01	441,0	12,17	123,8	858,2	2441	2447	2458	2437	2426	1737	2737	1607	2401	1525	1803	2890	2949	2761	2584	1834	1790	1667	767,3	1451	1705	1727	3172	2824
3	119,99	136,1	228,5	71,51	371,9	290,4	456,1	96,01	443,2	13,80	122,3	875,1	2426	2463	2465	2447	2428	1742	2740	1617	2405	1524	1815	2867	2979	2755	2605	1833	1789	1669	767,2	1456	1697	1715	3166	2833
4	179,97	137,7	229,1	71,35	373,7	293,6	457,9	95,18	448,9	13,07	118,0	879,7	2433	2458	2470	2447	2426	1730	2728	1604	2401	1521	1818	2866	2916	2747	2585	1847	1803	1662	757,4	1463	1718	1734	3173	2822
5	240,02	138,4	229,6	69,60	372,9	294,0	464,2	92,92	444,3	12,86	120,3	875,7	2431	2449	2478	2441	2422	1722	2736	1600	2401	1523	1810	2866	2966	2736	2609	1840	1793	1683	763,3	1449	1711	1720	3186	2824
6	300,53	130,5	229,7	69,35	372,4	286,9	456,8	95,36	439,2	11,59	117,9	874,3	2421	2451	2443	2426	2403	1706	2719	1587	2389	1516	1800	2859	2939	2747	2576	1819	1783	1650	752,8	1450	1685	1719	3155	2800
7	359,97	137,4	222,7	69,94	371,2	289,6	458,4	92,47	436,4	12,40	116,9	871,6	2425	2464	2456	2438	2408	1713	2722	1592	2396	1512	1780	2846	2948	2707	2563	1823	1778	1661	752,1	1463	1689	1724	3139	2797
8	419,98	131,9	228,3	70,30	369,2	285,1	456,3	91,63	438,7	11,92	113,2	875,0	2439	2446	2463	2435	2413	1716	2702	1590	2397	1510	1807	2879	2936	2715	2558	1817	1787	1656	752,9	1459	1700	1712	3143	2790
9	480,53	128,1	228,7	67,68	374,1	289,0	453,9	92,72	442,6	11,51	116,3	878,2	2437	2456	2465	2440	2420	1702	2701	1591	2386	1509	1795	2845	2899	2746	2552	1824	1780	1650	759,6	1448	1703	1719	3140	2816
10	539,97	133,5	229,0	65,33	362,9	289,8	454,7	90,12	439,1	12,59	115,0	880,1	2438	2458	2455	2423	2398	1700	2739	1587	2398	1511	1811	2836	2910	2716	2573	1827	1770	1662	752,1	1439	1696	1716	3162	2793
General																																				
General information																																				
																																				
	Session description																																			
	Software version			SkanIt Software 4.0 for Microplate Readers RE, ver. 4.0.1.136																																
	Optical response compensation			Yes																																
 																																				
Session information																																				
																																				
	Session name			26_05_2020_platefrom_20_05_2020																																
	Execution time			2020-05-26 09:40:10																																
	User			DESKTOP-8IGJT2P\biomatadm																																
 																																				
Instrument information																																				
																																				
	Name			Varioskan LUX																																
	ESW version			1.00.9																																
	Serial number			3020-052																																
																																				
	Instrument modules																																			
		Module's name		LAT module																																
		Module's serial number		LL151705																																
																																				
		Plate adapter name		96-well adapter for plate without lid																																
		Plate adapter number		2																																
																																				
		Incubator		Yes																																
		Gas control		No																																
		Top optics		Yes																																
		Bottom optics		Yes																																
		Dispenser		No																																
Protocol parameters																																				
																																				
	Measurement order			3																																
	Use settle delay			No																																
	Check temperature at start [C]			No																																
																																				
Kinetic Loop 1																																				
																																				
	No. of readings			10																																
	Kinetic Interval [hh:mm:ss]			00:01:00																																
	Shaking type			Continuous																																
	Shaking speed and force			Low																																
		Speed [rpm]		60																																
																																				
			Fluorescence 1																																	
																																				
				Wavelengths																																
					Excitation [nm] 		530																													
					Emission [nm] 		590																													
				Optics			Top																													
				Excitation bandwidth [nm]			5																													
				Dynamic Range			Automatic																													
				Measurement Time [ms]			100																													
																																				
Run log																																				
																																				
	Time	Event	Information																																	
	2020-05-26 09:40:12	Session 26_05_2020_platefrom_20_05_2020 started																																		
	2020-05-26 09:40:12	Temperature	25,1C																																	
	2020-05-26 09:40:13	Step Kinetic Loop 1 started																																		
	2020-05-26 09:40:13	Step Fluorescence 1 started																																		
	2020-05-26 09:40:18	Calibration	Fluorometric 1.23147 7028343																																	
	2020-05-26 09:40:19	Kinetic reading changed	Reading: 1 No. of readings: 10																																	
	2020-05-26 09:41:07	Temperature	25,1C																																	
	2020-05-26 09:41:19	Kinetic reading changed	Reading: 2 No. of readings: 10																																	
	2020-05-26 09:42:07	Temperature	25,1C																																	
	2020-05-26 09:42:19	Kinetic reading changed	Reading: 3 No. of readings: 10																																	
	2020-05-26 09:43:07	Temperature	25,1C																																	
	2020-05-26 09:43:19	Kinetic reading changed	Reading: 4 No. of readings: 10																																	
	2020-05-26 09:44:07	Temperature	25,1C																																	
	2020-05-26 09:44:19	Kinetic reading changed	Reading: 5 No. of readings: 10																																	
	2020-05-26 09:45:07	Temperature	25,0C																																	
	2020-05-26 09:45:17	Calibration	Fluorometric 1.22313 7058206																																	
	2020-05-26 09:45:19	Kinetic reading changed	Reading: 6 No. of readings: 10																																	
	2020-05-26 09:46:07	Temperature	25,1C																																	
	2020-05-26 09:46:19	Kinetic reading changed	Reading: 7 No. of readings: 10																																	
	2020-05-26 09:47:07	Temperature	25,1C																																	
	2020-05-26 09:47:19	Kinetic reading changed	Reading: 8 No. of readings: 10																																	
	2020-05-26 09:48:07	Temperature	25,1C																																	
	2020-05-26 09:48:19	Kinetic reading changed	Reading: 9 No. of readings: 10																																	
	2020-05-26 09:49:07	Temperature	25,1C																																	
	2020-05-26 09:49:19	Kinetic reading changed	Reading: 10 No. of readings: 10																																	
	2020-05-26 09:49:29	Step Fluorescence 1 ended																																		
	2020-05-26 09:49:29	Step Kinetic Loop 1 ended																																		
	2020-05-26 09:49:29	Temperature	25,1C																																	
	2020-05-26 09:49:37	Session 26_05_2020_platefrom_20_05_2020 ended																																		
 																																				
Name	Plate 1																																			
Plate template	Corning, clear bottom, white/black, half area, 96-well																																			
																																				
	1	2	3	4	5	6	7	8	9	10	11	12																								
A	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1																										
	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1																										
																																				
B	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1																										
	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1																										
																																				
C	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1	Blank1																										
	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1	Group 1																										
																																				
D	Blank1	Blank1	Blank1	Blank1	Blank1																															
	Group 1	Group 1	Group 1	Group 1	Group 1																															
																																				
E																																				
																																				
																																				
F																																				
																																				
																																				
G																																				
																																				
																																				
H																																				
																																				
																																				
																																				
																																				
																																				
																																				
 																																				
